Wave Bioreactor (Wave Bioreactor) is a kind of bioreactor widely used in the fields of biopharmaceuticals, cell culture and microbial fermentation. It utilizes the principle of fluctuation to form a circulating flow of culture solution in the reactor, thus improving the dissolved oxygen transfer efficiency and promoting cell growth. In this article, we will introduce the basic principles, maintenance points and operation skills of wave bioreactor in detail to help users better use and maintain the equipment. vessel, wave generator, gas distribution system, temperature control system and other components. Its working principle is as follows:

Vessel: the vessel of wave bioreactor is usually cylindrical with conical bottom, which is favorable for wave formation and dissipation.

Wave generator: driven by a motor, the wave generator produces waves to push the culture liquid to circulate in the reactor.

GAS DISTRIBUTION SYSTEM: Disperse the gases (e.g. oxygen, carbon dioxide) evenly into the culture liquid to improve the efficiency of dissolved oxygen transfer.

Temperature control system: keep the temperature of the culture liquid within a suitable range by means of a heating or cooling device.

Maintenance points of wave bioreactor

Equipment cleaning

(1) Daily cleaning: after every day's use, wipe the surface of the equipment with 70% alcohol to remove stains and bacteria.

(2) Regular Cleaning: Comprehensively clean the equipment once a month, including containers, piping, and gas distribution systems. Neutral detergent, ultrasonic cleaner and other tools can be used.

Checking and Replacing Parts

(1) Checking the Motor: Regularly check the motor running condition, if there is any abnormal sound, vibration, or temperature increase, it should be repaired or replaced in time.

(2) Replacement of seals: Regularly check the seals of containers, pipelines and other parts of the sealing ring, and replace them in time if there is any aging or wear and tear.

(3) Replacement of wave generator: According to the frequency of use, replace the wave generator regularly to ensure the wave effect.

Maintaining the gas distribution system

(1) Checking the gas pipeline: make sure that the gas pipeline is free of leakage and blockage.

(2) Replace the gas filter: Replace the gas filter regularly to prevent impurities from entering the culture fluid.

(3) Clean the gas distribution disk: clean the gas distribution disk regularly to ensure even distribution of gas.

Wave bioreactor operation tips

Control the temperature of the culture solution: according to the cell growth needs, the temperature of the culture solution will be controlled in the appropriate range, generally 36-38 ℃.

Adjust the gas flow: adjust the flow of oxygen and carbon dioxide according to the state of cell growth to maintain a suitable level of dissolved oxygen.

Testing the pH value of the culture medium: test the pH value of the culture medium regularly to ensure that it is within the appropriate range for cell growth.

Observation of cell growth: observe the cell morphology, density, etc. through microscope, and adjust the culture conditions in time.

Preventing contamination: Strictly aseptic operation, regular replacement of filters and sterilization equipment to reduce the risk of contamination.

In short, mastering the main points of maintenance and operation skills of wave bioreactor can help to improve the success rate of cell culture, and provide a strong support for the fields of biopharmaceuticals, scientific research and so on! . Users should pay attention to details during the use process to ensure stable equipment performance and create a good environment for cell growth.