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Discussion on the application and maintenance points of wave bioreactor in biopharmaceutical production
2024-11-15

Wave bioreactor in biopharmaceutical production and maintenance points to explore

Wave bioreactor has a wide range of applications in the field of biopharmaceuticals, which provides a highly efficient and stable cultivation environment for cell culture, protein expression, and vaccine production. In this paper, we will focus on the application of wave bioreactor in biopharmaceutical production and its maintenance points, aiming to provide reference for related enterprises and research institutions.


Application of wave bioreactor in biopharmaceutical production

Cell culture

Wave bioreactor provides a favorable environment for cell growth through its unique wave motion. In biopharmaceutical production, it is commonly used for the cultivation of mammalian cells, insect cells, microbial cells and many other cells, laying the foundation for the production of antibodies, recombinant proteins and other biopharmaceuticals.

Protein expression

Wave bioreactors are capable of high-density cell culture, which is conducive to the improvement of protein expression. By optimizing the culture conditions, the yield and quality of target proteins can be further improved to meet the demands of biopharmaceutical production.

Vaccine production

Wave bioreactors play an important role in vaccine production, such as influenza vaccine and neocoronazole vaccine. It can realize large-scale cell culture, improve vaccine production efficiency and reduce production cost.

Maintenance points of wave bioreactor are discussed

Ensuring aseptic operation

Aseptic operation is crucial in biopharmaceutical production. Maintenance points of the wave bioreactor are as follows:

(1) Strict disinfection: use highly effective disinfectants to thoroughly disinfect the equipment to ensure that there is no microbial contamination such as bacteria and viruses.

(2) Aseptic inspection: Regularly conduct aseptic inspection of the equipment to ensure that the culture environment meets the requirements.

Optimization of operating parameters

(1) Dissolved oxygen control: Adjust the level of dissolved oxygen according to the demand of cell growth to ensure the normal metabolism of cells.

(2) pH adjustment: real-time monitoring of the pH value of the culture medium, adjusting it by adding acid-base solution to maintain a suitable acid-base environment.

(3) Temperature control: Ensure the temperature in the reactor is stable to meet the demand of cell growth.

Equipment Inspection and Maintenance

(1) Daily Inspection: Check the operating status of the equipment, including the motor, wave generator, gas system, etc., to ensure normal operation.

(2) Regular Maintenance: According to the frequency of equipment use, carry out regular maintenance, including replacing wearing parts, cleaning filters and so on.

(3) Troubleshooting: When encountering equipment failures, promptly investigate the causes and take appropriate measures to solve the problem.

Culture solution management

(1) Quality control: Ensure the quality of the culture solution is qualified, free of contamination, and with sufficient nutrients.

(2) Replenishment strategy: supplement nutrients and adjust the composition of the culture medium in time according to cell growth.


In short, the application of wave bioreactor in biopharmaceutical production and its maintenance points are the key to ensure smooth production. By strengthening aseptic operation, optimizing operating parameters, equipment inspection and maintenance, and culture solution management, the use of wave bioreactors can be improved to create greater value for the biopharmaceutical industry. In the actual production process, enterprises should continuously improve the maintenance and management system according to their own situation to improve production efficiency.

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