Biopharmaceutical industry is one of the most promising industries in the 21st century, and our government has also listed it as a strategic emerging industry. In the process of biopharmaceutical production, bioreactor is one of the key equipments. wave bioreactor, as a new type of bioreactor, has been widely used in biopharmaceutical industry with its unique advantages. In this paper, we will introduce the principle, characteristics and application of wave bioreactor in biopharmaceutical industry in detail.

II, Principle and Characteristics of wave bioreactor

1. Principle

wave bioreactor adopts wave stirring method, so that the culture liquid forms wave-like flow in the reactor. This type of flow is favorable to the full contact between cells and nutrients, increasing the cell growth rate and product concentration. At the same time, the wave-like flow can also effectively reduce the shear force and minimize cell damage.

2. Features

(1) Simple structure: wave bioreactor adopts a single-tank design, which eliminates the need for a complex control system, and is easy to operate and maintain.

(2) Low shear: wave stirring method makes the shear force much lower than traditional stirred bioreactor, which is favorable for cell growth and product expression.

(3) High oxygen transfer efficiency: wave bioreactor adopts a gas injection device, which makes the gas bubbles form tiny bubbles in the liquid and improves the oxygen transfer efficiency.

(4) Small footprint: the single tank design makes wave bioreactor cover a small area, saving space.

(5) Small amplification effect: wave bioreactor has a small amplification effect, which is conducive to the realization of large-scale production.

III. Applications of wave bioreactor in the biopharmaceutical industry

1. Cell culture

wave bioreactor is suitable for animal cells, plant cells, plant cells and microbial cells. It is widely used in cell therapy, vaccine production and other fields.

(1) Animal cell culture: wave bioreactor can be used for the production of virus vaccines, monoclonal antibodies and other biological products. For example, a number of domestic and foreign companies utilize wave bioreactor to produce influenza vaccine, HPV vaccine and so on.

(2) Plant cell culture: wave bioreactor can be used for the production of plant extracts, secondary metabolites and so on. For example, wave bioreactor is utilized to culture the red bean sprouts cells to produce paclitaxel.

(3) Microbial cell culture: wave bioreactor is suitable for fermentation to produce antibiotics, enzymes and so on. For example, wave bioreactor is utilized for the production of penicillin, insulin, and so on.

2. Protein engineering

wave bioreactor can be used for protein engineering research, such as directed evolution, protein expression optimization. By adjusting the culture conditions, the expression and activity of target proteins are improved.

3. Tissue engineering

wave bioreactor can be used for tissue engineering research, such as scaffolding material preparation, three-dimensional culture of cells. It promotes cell growth and differentiation by simulating the in vivo environment.

IV. Advantages of wave bioreactor

1. Improvement of production efficiency: wave bioreactor has high cell density and product concentration, which is conducive to the improvement of production efficiency.

2. Reduce production cost: wave bioreactor simplifies the production process and reduces the investment in equipment and operation cost.

3. Stabilize product quality: wave bioreactor reduces the damage of shear force on cells, which is helpful to maintain product activity.

4. Easy industrialization: wave bioreactor has a small amplification effect, which is conducive to the realization of large-scale production.

V. Summarization

wave bioreactor, as a new type of bioreactor, has a wide range of application prospects in the biopharmaceutical industry. With the rapid development of China's biopharmaceutical industry, wave bioreactor will play an increasingly important role in the fields of vaccine production, cell therapy, protein engineering and so on. To further improve the performance of wave bioreactor, future research can focus on the following aspects:

1. Optimize the wave stirring method to improve the oxygen transfer efficiency.

2. Research on novel gas injection devices to reduce the damage of gas bubbles to cells.

3. Explore the application of wave bioreactor in the production of other biologics.

4. Conducting industrialization research of wave bioreactor to promote the development of biopharmaceutical industry.