Bioreactor is an indispensable equipment in the field of bioengineering, which provides a suitable environment for cell culture and ensures the production efficiency and safety of biologics. As a new type of cell culture equipment, the swing bioreactor, with its unique advantages and stable performance, has been widely used in the fields of cell therapy, antibody drug seed expansion and vaccine production. In this paper, we will discuss in detail the design principle, operation characteristics of swing bioreactor and its application in various fields.

The design and principle of the rocking bioreactor {{20 21}}

1. Design features

Swinging bioreactors are designed specifically for cell culture, and have the following main features:

• Disposable reaction bag: The single-use design reduces the risk of cross-contamination and ensures the safety of the production process.
• Parameter control: It is able to accurately control key parameters such as rocking speed, rocking angle, gas flow rate, pH, dissolved oxygen (DO), temperature and so on, to provide an optimal environment for the growth of cells.

2. Working Principle

Swinging bioreactor simulates the growth environment of cells in the body through the swaying motion, so that the cells are evenly distributed in three-dimensional space. uniformly distributed in three-dimensional space, increasing the contact area between cells and nutrients, thus promoting cell growth and metabolism.

Operation and control of the swinging bioreactor

1. Operation flow

Operation flow of the swing bioreactor includes the following steps:

• Reaction bag preparation: the disposable reaction bag is mounted onto the reactor and sterilized.
• Parameter setting: according to the needs of the cell culture, set the parameters of rocking speed, rocking angle, gas flow rate, pH, DO, and temperature.
• Cell inoculation : Inoculate the cell suspension into the reaction bag to start the culture process.
• Monitoring and adjustment: real-time monitoring of the parameters in the cultivation process, and adjusting the parameters according to the cell growth.
• Harvesting cells: after the cell culture is completed, take out the reaction bag for cell harvesting or further processing.

2. Control system

Control system of the swinging bioreactor Mainly include:

• Swinging control system: realizes three-dimensional cell culture by adjusting the speed and angle of swinging.
• Gas flow control system: accurately control the flow of oxygen and carbon dioxide to maintain the stability of the culture environment.
• pH and DO monitoring system: real-time monitoring and adjustment of pH and DO values to ensure the acidity and oxygen supply required for cell growth.
• Temperature control system: maintains the temperature constant during the culture process, avoiding the effect of temperature fluctuation on cell growth.

Swinging bioreactors in cellular therapy