Biopharmaceutical industry is a strategic emerging industry that China focuses on the development of, bioreactor as the core equipment in the process of biopharmaceuticals, the improvement of its technical level is crucial for the development of the whole industry. Wave bioreactor as an innovative bioreactor technology, its application in the field of biopharmaceuticals is receiving increasing attention. In this paper, we will discuss in depth the principle, characteristics and practical application of wave bioreactor in the biopharmaceutical industry.

1.Principles and characteristics of wave bioreactor

Principles

The wave bioreactors are designed to produce a wave-like flow of the culture liquid by installing a wave generating device at the bottom of the reactor, thus realizing the mixing of the culture liquid and the transfer of oxygen.

Features

(1) Closed system: The wave bioreactor adopts a closed design, which effectively reduces the risk of external contamination and ensures the safety of the production process.

(2) Easy operation: Compared with traditional bioreactors, the wave bioreactor is easier to operate, without the need for a complex control system, which reduces production costs.

(3) Low shear: the wave flow makes the shear force distribution more uniform, which is favorable for cell growth and metabolism.

(4) High oxygen transfer efficiency: the wave flow helps to improve the oxygen transfer efficiency and meets the oxygen demand of cells in high-density culture.

(5) High space utilization: Wave bioreactor is compact and covers a small area, which is suitable for space layout in laboratory and industrial production.

2.Application of wave bioreactor in biopharmaceutical industry

Cell culture

The application of wave bioreactor in cell culture significantly improves the density of cell growth and the level of product expression. For example, in the production of recombinant proteins and antibodies, the wave bioreactor showed high yield and stable product quality.

Microbial fermentation

In microbial fermentation, wave bioreactors can improve fermentation efficiency and reduce production costs, and are suitable for the production of microbial products such as antibiotics and enzymes.

Tissue engineering

Wave bioreactor is of great value in the field of tissue engineering, which can simulate the in vivo environment for the cultivation of cardiomyocytes, hepatocytes, and other cells, and provides effective technical support for tissue engineering research.

Vaccine production

In vaccine production, wave bioreactors have demonstrated the advantages of a short production cycle and high yield, and have been widely used in the production of influenza vaccines, new crown vaccines, etc.

Wave bioreactors, as a kind of advanced bioreactor technology, have demonstrated significant advantages in their application in the field of biopharmaceuticals. With the continuous development of the biopharmaceutical industry, wave bioreactor is expected to play a greater role in the future production practice and provide strong support for the innovation and development of China's biopharmaceutical industry.