Wave Bioreactor (Wave Bioreactor), as an advanced bioreactor, has a wide range of applications in the biopharmaceutical field. In this paper, the working principle, advantages of Wave Bioreactor and its application in the biopharmaceutical industry will be introduced in detail to provide useful references for biopharmaceutical practitioners.

The biopharmaceutical industry is one of the industries with the most promising development potential in the 21st century, in which the bioreactor is the As a new type of bioreactor, Wave Bioreactor has emerged in the biopharmaceutical field with its unique advantages. This paper will focus on the principle of Wave Bioreactor and its application in biopharmaceutical industry.

Principle

1. Principle of operation

Wave BioreactorAdopts the wave stirring technology, and through the gas injection device Waves are generated so that the culture liquid forms a circulating flow in the reactor. This kind of flow is conducive to the full contact between cells and nutrients, and improves the cell growth rate and product expression level. The main working principles are as follows:

(1) Gas injection device: gas (e.g., oxygen, carbon dioxide) is injected into the culture fluid to form bubbles.

(2) Wave generation: during the rise of bubbles, the culture fluid is driven to produce waves.

(3) Circulation flow: the waves make the culture liquid form a circulation flow in the reactor, realizing the full mixing of cells and nutrients.

2. Structural features

(1) Single-tank design: single-tank design is adopted, which simplifies the structure of the equipment and reduces the risk of cross-contamination.

(2) No stirring device: Compared with the traditional stirred bioreactor, no stirring device is required, which reduces equipment failure and maintenance costs.

(3) Uniform gas distribution: the gas injection device makes the gas uniformly distributed in the reactor, which is favorable for cell growth.

Advantages

1. High cell density: Wave bioreactor can realize high cell density culture and improve the product expression level.

2. Low shear: Wave stirring technology reduces the shear force, which is favorable for cell growth and product expression.

3. Easy to scale up: The single-tank design of the wave bioreactor makes it easy to scale up for industrial production.

4. Space-saving: the equipment covers a small area, saving production space.

5. Easy operation: high degree of automation, easy operation, reducing production costs.

Applications in the biopharmaceutical industry

1. Antibody production: Widely used in the production of antibody drugs, such as tumor immunotherapy drugs, autoimmune disease therapeutic drugs.

2. Vaccine production: It can be used for the production of viral vaccines, such as influenza vaccine, neocoronary vaccine and so on.

3. Cell therapy: can be used for the production of cell therapy products, such as CAR-T cells, stem cell therapy, etc.

4. Recombinant protein production: can be used for the production of recombinant protein drugs, such as insulin, growth hormone and so on. has a wide range of application prospects. Its unique wave stirring technology provides a favorable growth environment for cell culture and improves the product expression level. With the continuous development of the biopharmaceutical industry, Wave Bioreactor will play a greater role in the future. China's biopharmaceutical enterprises should actively introduce and develop Wave Bioreactor technology to enhance the competitiveness of China's biopharmaceutical industry.