Bioreactor is the key equipment in the biopharmaceutical industry, and its role is to provide a suitable growth environment for the cells to realize the mass production of biological products. In this paper, the working principle of bioreactor and its application in biopharmaceutical industry will be introduced in detail.

Biopharmaceuticals It refers to the drugs produced by means of biotechnology, which are characterized by good efficacy and low side effects. With the continuous development of biotechnology, the biopharmaceutical industry has developed rapidly in China. As the key equipment of biopharmaceutical industry, the performance of bioreactor directly affects the production efficiency and quality of biological products. This paper will focus on the working principle of bioreactor and its application in the biopharmaceutical industry.

Working principle of bioreactor

1. Basic concept of bioreactor

Bioreactor is a kind of equipment used in the production of bioproducts, and its main function is to provide cells with a suitable growth environment in which the cells can reproduce and metabolize to achieve the production of the target product.

2. Working Principle of Bioreactor

(1) Cell Cultivation: The bioreactor firstly needs to inoculate the cells into the culture medium, which will provide nutrients and space for the cells to grow.

(2) Dissolved Oxygen Control: The bioreactor keeps the concentration of dissolved oxygen in the culture medium within a suitable range by adjusting parameters such as stirring speed, aeration, and temperature to meet the needs of cell growth.

(3) pH control: the bioreactor keeps the pH of the culture medium in a suitable range by adding acid-base regulator or using membrane electrode control system.

(4) Temperature control: the bioreactor keeps the culture temperature in the optimal range for cell growth by means of a heating or cooling device.

(5) Metabolite monitoring: the bioreactor monitors the cell's metabolites, such as glucose and lactate, in real time in order to adjust the culture conditions.

(6) Cell harvesting: when the cells multiply to a certain density or the target products reach the expected yield, the cells and products are separated by centrifugation and filtration.

Bioreactors in the biopharmaceutical industry

1. Production of vaccines

Bioreactors play an important role in vaccine production. Taking influenza vaccine as an example, the influenza vaccine produced by bioreactor has the advantages of high purity and good safety.

2. Production of Antibody Drugs

Antibody drugs are an important part of the biopharmaceutical industry, and bioreactors play a key role in antibody drug production. Through genetic engineering technology, antibody genes are introduced into cells, and bioreactors are utilized to produce antibody drugs on a large scale.

3. Production of recombinant protein drugs

Recombinant protein drugs have a wide range of applications, such as insulin and growth hormone. Bioreactors provide an effective means for the production of recombinant protein drugs.

4. Production of cell therapy products

Cell therapy is an emerging field in the biopharmaceutical industry, and bioreactors play an important role in the production of cell therapy products. For example, stem cells and immune cells need to be cultured and expanded in bioreactors.

Bioreactors, as the key equipment in the biopharmaceutical industry, have an important significance in terms of their working principle and their application in the biopharmaceutical industry. With the continuous development of biotechnology, the bioreactor will be continuously improved to provide strong support for the development of China's biopharmaceutical industry.