Cell culture, as an important technical means in the fields of bioengineering, biomedicine and other fields, is widely used in scientific research, drug research and development, clinical treatment and other aspects. With the rapid development of biotechnology, higher requirements have been put forward for cell culture technology. Traditional cell culture methods have certain limitations in terms of operation complexity, production scale and cost control. In recent years, wave bioreactor technology, as a new type of cell culture technology, has gradually received widespread attention. In this paper, wave bioreactor technology and its application in cell culture field will be introduced in detail.

Overview

1. Principle of the technology

The Wave Bioreactor technology is a cell culture technology based on wave agitation. The technology is based on the installation of a wave agitator at the bottom of the culture vessel, which creates a wave-like flow of the culture solution, thus realizing a thorough mixing of cells and nutrients. Compared with traditional stirred bioreactors, Wave Bioreactor has the following advantages:

(1) gentle stirring process with little damage to cells;

(2) high oxygen mass transfer efficiency, which is favorable to cell growth;

(3) Small footprint, convenient for laboratory and industrialized production;

(4) Simple operation, high degree of automation.

2. Composition of the equipment

mainly consists of the following parts:

(1) Culture vessel: generally disposable, made of plastic or glass;

(2) Wave stirrer: located at the bottom of the vessel, responsible for generating wave-like flow;

(3) Control system: including temperature, pH, dissolved oxygen and other parameters monitoring and regulation;

(4) Gas supply system: to provide the necessary oxygen and carbon dioxide for cell growth.

Applications in the field of cell cultivation

1. animal Cell Culture

Wave Bioreactor technology has a wide range of applications in the field of animal cell culture, such as vaccine production and biopharmaceuticals. The following is an example of the application of Wave Bioreactor technology for vaccine production.

(1) Virus vaccine production: using Wave Bioreactor technology, high-density cultivation of virus-producing cells can be realized to improve virus yield. For example, the production of influenza virus, adenovirus and other vaccines;

(2) Recombinant Protein Vaccine Production: Through genetic engineering technology, the target genes are introduced into the cells, and high density culture is carried out by utilizing the Wave Bioreactor technology, which can achieve the high efficiency of the recombinant protein expression.

2. Plant cell culture

Wave Bioreactor technology in plant cell culture is mainly applied in the following aspects:

(1) Plant cell mass culture: through the Wave Bioreactor technology, the high-density cultivation of plant cells is realized, which can provide raw materials for the production of plant secondary metabolites;

(2) Plant genetic transformation: Utilizing Wave Bioreactor technology to improve the transformation efficiency of plant cells and provide technical support for genetic engineering breeding.

3. Microbial cell culture

The main applications of Wave Bioreactor technology in microbial cell culture include:

(1) Microbial fermentation: High density fermentation of microorganisms is realized by Wave Bioreactor technology to increase the yield of fermentation products;

(2) Microbial transformation: using Wave Bioreactor technology to improve the efficiency of microbial transformation and provide an optimized solution for biocatalytic processes.

Trends and Prospects

With the continuous advancement of bio technology continues to advance, the development of Wave Bioreactor technology in the field of cell culture has shown the following trends:

1. Equipment miniaturization and portability: easy to operate in the laboratory and on-site, reducing production costs;

2. Process intelligence and automation: improve production efficiency and reduce human error;

3. Expanded application: from biopharmaceuticals, vaccine production and other fields to more bioengineering fields.

In conclusion, Wave Bioreactor technology has a broad application prospect in the field of cell culture. China should increase the research and development efforts to promote the innovative development of Wave Bioreactor technology in the field of bioengineering in China.