Laboratory size

Laboratory scale -
(Glass bioreactor/Fermentation system）

The laboratory series bioreactor/fermentation tank system under Jichen Bio-tech is a set of reaction systems that are flexible in combination, comprehensive in function, and easy to operate, It can be used for batch, flow addition, continuous/perfusion cultivation processes, and integrates online glucose/lactate detection and exhaust gas analysis, By using magnetic mechanical stirring combined with servo motors, the stirrer can be sterilized as a whole with the glass tank, avoiding bacterial contamination. It can be used for various cell/microbial cultures, And the software is localized, with a high user-friendly interface. Widely used in research and development centers such as synthetic biology/monoclonal antibody/vaccine companies, as well as laboratories in universities/science centers.

Performance parameter

Cell culture
Model		500ml	3L	5L	7L	10L	15L
Tank body	Total volume	538	3100	4800	6800	10000	16500
Tank body	Working volume	100-400	1000-2700	1500-3400	1500-5000	3000-7000	4500-10000

Microbial and algal cultivation
Model		500ml	3L	5L	7L	10L	15L
Tank body	Total volume	500	3170	5000	7460	10000	16500
Tank body	Working volume	100-400	1000-2100	1500-3500	1500-5000	3000-7000	4500-10000

Case data presentation

Project background: Testing whether the composition of the culture medium in the drug production process of the enterprise meets the growth needs of CHO cells

Purpose of operation:

(1) Test the control effect of cultivation temperature, pH, and DO parameters in glass bioreactors
(2) Testing the cell culture effect of glass bioreactors

Facilities and processes:

(1) Facilities: 2L glass bioreactor, CHO cells
(2) Cultivation parameter settings: Set appropriate pH, temperature, and dissolved oxygen content, and adjust blade speed.
(3) Operation process: Cells are inoculated into a bioreactor, cultured according to the culture parameters, and sterile samples are taken every day. Key parameters such as cell density, vitality, and glucose are measured offline, and continuous cultivation is carried out.

Data and Effect Display:

During the cultivation period, the temperature control deviation of the glass reactor was ± 0.1, and the DO control deviation was 1%, indicating good control effect.

Project Summary:

The key parameter control accuracy of the 2L glass bioreactor is high, and the cell culture is normal, fully meeting the process requirements of users. The cell culture status during the process is similar to the shaking bottle culture data, making it suitable for exploring the culture process. The pH control range is broad, Adjustable, reducing frequent user operations on set values and reducing the entry of CO2 and alkaline solution.